CaMKII alpha/beta (Internal Region) antibody

Catalog No. ABIN570984

This anti- antibody is a Goat Polyclonal antibody detecting in WB, ELISA, FACS and IF. Suitable for Rat, Human and Mouse.

Quick Overview for CaMKII alpha/beta (Internal Region) antibody (ABIN570984)

Target: CaMKII alpha/beta

Reactivity: Rat, Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details

Binding Specificity: Internal Region

Purpose: CAMK2

Sequence: PRTAQSEETR VWHR

Specificity: This antibody is expected to recognize both reported isoforms (NP_057065.2 (CAMK2A) and NP_001280099.1 (CAMK2B).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2, NP_741960.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48-50+60 kDa bands observed in Human Cerebral Cortex and Mouse and Rat Brain lysates, corresponding to CAMK2alpha and CAMK2beta respectively and approx. 60 kDa in Human Cerebellum lysates (calculated MW of 55.3 kDa according to Human NP

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of Neuro2a cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Neuro2a cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details

Target: CaMKII alpha/beta

Alternative Name: CAMK2A/B

Background: CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase

Gene ID: 815, 816, 12322

NCBI Accession: NP_057065, NP_741960